п»їLab Report #6
Electrophoresis: Using Electrical energy to Separate Molecules
Answer the next questions regarding the results of the digital lab activity. Record your answers inside the boxes.
Activity 1 вЂ“ Calibration
Record your data by Activity 1 in the boxes below. Your data (number of groups, description with the band patterns) you gathered for the protein step ladder in the appropriate columns for the skin gels concentrations (8%, 10%, 12%)
Number of Rings
Explanation of Music group Patterns
(weights, relative sizes, and spacing)
1 ) Which percent thickness skin gels shows the most ladder groups?
2 . Illustrate the relationship between the thickness with the gel plus the distribution from the ladder artists.
Activity a couple of
Record your details from Activity 2 in the boxes below. Enter the info (number of bands, approximate weight of band, space pattern of bands) you collected intended for the trials (fish, crab, food, protein ladder) inside the appropriate articles. The approx . molecular excess weight of a group is determined by comparing the strap of the foodstuff sample for the protein step ladder bands.
Protein Ladder (M)
Quantity of Bands
Weight of the Artists
Spacing Pattern of Bands
1 separated from two pair of groups spaced apart
2 artists at 45 and up to 29 kd
2 bands in 45 and 29 in pieces
Dark artists spread out along each in pieces measurement
3. Explain if the food claim around the Crab Meat is true or not.
4. How does the ladder help with distinguishing involving the fish muscle tissue protein plus the crab muscles protein?
your five. What is the approximate weight in kD of the music group that is exceptional to crab muscle necessary protein and is not found in abundance in seafood?
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